| 基于2-二氰基亚甲基-3-氰基-4，5，5-三甲基-2，5-二氢呋喃近红外硫化氢荧光探针的构筑及活细胞成像 |
| A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells |
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| 摘要: 使用2-二氰基亚甲基-3-氰基-4，5，5-三甲基-2，5-二氢呋喃(TCF)作为近红外荧光发色团，通过引入2，4-二硝基苯醚作为H2S的识别位点，我们设计和合成了一种基于TCF的荧光探针TCF-NS。该探针对2S显示出快速颜色和荧光响应，具有高度的灵敏度和选择性，但对其他生物巯基没有明显的荧光响应。此外，TCF-NS已成功应用于检测活细胞中的2S。 |
| 关键词: 硫化氢 近红外荧光探针 细胞成像 |
| 基金项目: 江苏省自然科学基金(No.BK20241181)和南京大学化学化工学院生命分析化学国家重点实验室(No.SKLACLS2419)资助。 |
| Abstract: Using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) as a near-infrared fluorescent chromophore, we designed and synthesized a TCF-based fluorescent probe TCF-NS by introducing 2,4-dinitrophenyl ether as the recognized site for H2S. The probe TCF-NS displayed a rapid-response fluorescent against H2S with high sensitivity and selection but had no significant fluorescence response to other biothiols. Furthermore, TCF-NS was applied to sense H2S in living cells successfully with minimized cytotoxicity and a large Stokes shift. |
| Keywords: hydrogen sulfide near-infrared fluorescence probe cell imaging |
| 投稿时间：2024-11-12 修订日期：2024-12-16 |
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| 张林芳,尹文竹,尹桂.基于2-二氰基亚甲基-3-氰基-4，5，5-三甲基-2，5-二氢呋喃近红外硫化氢荧光探针的构筑及活细胞成像[J].无机化学学报,2025,41(3):540-548. |
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