| 氮掺杂荧光碳点用于Co2+的超灵敏检测及细胞成像 | |
| Nitrogen-doped fluorescent carbon dots are used for ultra-sensitive detection of Co2+ and cell imaging | |
| 摘要: 首次以金银花和乙二胺为原料，通过水热法合成出性能优异的氮掺杂碳点(N-CDs)。制备的N-CDs具有丰富的官能团、良好的水溶性、低细胞毒性、高的荧光稳定性和良好的生物相容性。在最佳条件下，N-CDs能够高选择性地检出Co2+，N-CDs的荧光强度在0.5~3.6 nmol·L-1范围内被Co2+线性猝灭，检出限低至1.38 nmol·L-1，其猝灭机制属于内滤效应和静态猝灭。该方法也已成功应用于实际样品的精确分析。此外，N-CDs还可用于细胞成像及细胞内Co2+传感。 | |
| 关键词: 氮掺杂碳点 Co2+ 水热法 传感 细胞成像 | |
| 基金项目: 山西省基础研究计划青年项目(No.202103021223324，202103021223326，202103021223333)和山西省基础研究计划面上项目(No.20210302123089)资助。 | |
| Abstract: For the first time, nitrogen-doped carbon dots (N-CDs) with excellent properties were synthesized by hydrothermal method using honeysuckle as a carbon source and ethylenediamine as a nitrogen source. The prepared N-CDs have abundant functional groups, small particle sizes, good water solubility, excellent fluorescence performance, and high chemical stability. They can emit bright blue fluorescence under ultraviolet light. Using quinine sulfate as a reference, the fluorescence quantum yield of N-CDs was measured to be as high as 15.6%. Under optimal conditions, N-CDs could selectively detect Co2+, and the fluorescence intensity of N-CDs was linearly quenched by Co2+ in a range of 0.5 to 3.6 nmol·L-1, with a detection limit as low as 1.38 nmol·L-1. The quenching mechanism belongs to the internal filtering effect and static quenching. Therefore, an ultra-sensitive Co2+ detection method has been established. To further confirm the feasibility of this sensor, we have also successfully applied it to the precise analysis of Co2+ in tap water samples. In addition, N-CDs have been successfully used for cell imaging and intracellular Co2+ sensing due to their low cytotoxicity and good biocompatibility. | |
| Keywords: nitrogen-doped carbon dots Co2+ hydrothermal method sensing cell imaging | |
| 投稿时间：2023-04-08 修订日期：2023-06-26 | |
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| 温香平,沈腊珍,李建会,高晓丽,李轶坤,张鑫,吕佳玉.氮掺杂荧光碳点用于Co2+的超灵敏检测及细胞成像[J].无机化学学报,2023,39(8):1471-1480. | |
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