| 超快速turn-on型ClO-荧光探针的合成及性能 |
| Synthesis and Performance of a Rapid Turn-On Fluorescent Probe for ClO- |
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| 摘要: 为实现次氯酸根的实时、快速、便捷检测，我们以2，7-二溴-9-芴酮为原料，通过两步常规反应，合成了一种新型的turn-on型ClO-荧光探针。在磷酸缓冲盐溶液（PBS）中，随着ClO-浓度的增大，探针在511 nm的发射峰逐渐增大，呈绿色荧光发射。探针与ClO-反应迅速，10 s内反应完成。此外，该探针对ClO-有较低的检出限，检出限为0.74 μmol·L-1。细胞实验表明探针可以在细胞水平检测内源性和外源性的ClO-。 |
| 关键词: turn-on型 次氯酸根 荧光探针 细胞成像 |
| 基金项目: 山西省高等学校教学改革创新项目（No.J2021720）资助。 |
| Abstract: We synthesized a new type of ClO- fluorescence probe using 2,7-dibromo-9-fluorenone as raw material by two-step conventional reaction. In phosphate buffered saline (PBS), with the increase of ClO-, the emission of the probe at 511 nm gradually enhanced, presenting green fluorescence emission. The probe reacted with ClO- quickly and the reaction was completed within 10 s. In addition, the present method exhibited high sensitivity to ClO- with a limit of detection of 0.74 μmol·L-1. Cell image experiments showed that the probe could detect endogenous and exogenous ClO- at the cellular level. |
| Keywords: turn-on type hypochlorite fluorescent probe cell imaging |
| 投稿时间：2021-09-30 修订日期：2021-11-14 |
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| 刘艳玲.超快速turn-on型ClO-荧光探针的合成及性能[J].无机化学学报,2021,37(12):2244-2248. |
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| Support information: 相关附件： file210447_210447-Supporting_Information.doc |
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