| 一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用 |
| A Fluorescence-Enhanced Probe Based on Benzimidazole for Bisulfite and Its Practical Application |
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| 摘要: 合成和表征了一种用于检测HSO3-的苯并咪唑类荧光增强型探针（FP1）。结果表明：FP1对HSO3-表现出了明显的荧光关-开响应，具有高的选择性和灵敏度，且抗干扰能力强，响应时间短（2 min）。FP1的荧光强度与HSO3-的浓度在1.2~8.1 μmol·L-1的范围内呈线性关系，其对HSO3-的检出限为0.14 μmol·L-1。此外，FP1能用于实际样品中HSO3-的检测，回收率良好，它还可应用于HeLa活细胞中对HSO3-进行荧光成像。 |
| 关键词: 亚硫酸氢根 荧光探针 苯并咪唑 检测 荧光成像 |
| 基金项目: 国家自然科学基金（No.51372077）资助项目。 |
| Abstract: A benzimidazole-based fluorescent turn-on probe (FP1) for the detection of HSO3- has been synthesized and characterized. The results showed that FP1 exhibited a clear off-on fluorescence response toward HSO3-, and it was highly selective and sensitive to HSO3- with strong anti-interference and a short response time (2 min). The fluorescence intensity of FP1 varied linearly with the concentration of HSO3- in the range of 1.2~8.1 μmol·L-1, and the detection limit for HSO3- was estimated to be 0.14 μmol·L-1. In addition, FP1 can be used to detect HSO3- in real samples with good recovery, and it was also applicable for fluorescence imaging of HSO3- in living HeLa cells. |
| Keywords: bissulfite fluorescent probe benzimidazole determination fluorescent imaging |
| 投稿时间：2019-09-12 修订日期：2019-12-23 |
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| 王成,杨曼,邓祥义,何明礼.一种检测亚硫酸氢根的苯并咪唑类荧光增强型探针及其实际应用[J].无机化学学报,2020,36(4):762-768. |
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| Support information: 相关附件： file190462_190462_Supporting_Information(revised).docx |
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