| 荧光介孔二氧化硅纳米粒子的合成及药物运输 | |
| Synthesis of Fluorescent Mesoporous Silica Nanoparticles and Application for Intracellular Drug Delivery | |
| 摘要: 合成了荧光介孔二氧化硅纳米粒子（MSNs-FITC），并研究了其在持续药物释放和生物示踪成像方面的应用。首先，采用一步法合成出MSNs-FITC，结合SEM、TEM、FT-IR、XRD和氮气吸附脱附等表征技术进行表征。其次，将抗癌药物阿霉素（DOX）负载到MSNs-FITC中。载药粒子的药物释放行为具有明显的pH依赖性，酸性环境加速释放速率。同时，体外细胞毒性测试表明MSNs-FITC具有良好的生物相容性。激光共聚焦扫描显微镜（CLSM）图像表明，MSNs-FITC可以进入细胞并具有剂量依赖性，流式细胞术分析（FCM）进一步证明了这一结果。 | |
| 关键词: 介孔二氧化硅纳米载体 药物运输 细胞毒性 生物成像 | |
| 基金项目: 中央高校基本科研业务费专项资金（No.2018BSCXB11）和江苏省研究生科研与实践创新计划项目（No.KYCX18_1937）资助 | |
| Abstract: Here, we reported the synthesis and FITC-functionalized of mesoporous silica nanoparticles (MSNs) for tracing bioimaging and sustained drug release. Firstly, the MSNs-FITC were synthesized through one-pot method and characterized by SEM, TEM, FT-IR, XRD and the N2 adsorption-desorption isotherm respectively. Then a classic anticancer drug doxorubicin (DOX) was loaded into the MSNs-FITC. The DOX-loaded nanocarriers exhibited obviously pH dependent release behavior with accelerated release rate in acidic environment. Meanwhile, the in vitro cytotoxicity evaluation exhibited that the nude MSNs-FITC were highly biocompatible. Furthermore, the confocal laser scanning microscope (CLSM) images indicated that MSNs-FITC could penetrate into living cells effectively and dose-depended internalization of MSNs-FITC were further investigated by flow cytometry analysis (FCM). | |
| Keywords: mesoporous silica nanocarriers drug delivery cytotoxicity bioimaging | |
| 投稿时间：2019-08-13 修订日期：2019-10-04 | |
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| 陈敏敏,耿浩然,胡金霞,张琼,Godfred Amfo Agyekum,张卓琦,曹希传.荧光介孔二氧化硅纳米粒子的合成及药物运输[J].无机化学学报,2019,35(11):2125-2135. | |
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