| 4-(2-羟基-3-氯)苯基-2,2':6',2"-三联吡啶Cu(Ⅱ)配合物的合成、结构表征及抗肿瘤活性 | |
| Synthesis, Structural Characterization and Antitumor Activity of Copper(Ⅱ) Complex of 4-(2-Hydroxy-3-chlorine)phenyl-2,2': 6',2'-terpyridine | |
| 摘要: 以4-（2-羟基-3-氯）苯基-2，2'：6'，2"-三联吡啶（HL）为配体，合成了一种新的铜（Ⅱ）配合物[Cu2（μ-L-κO，O）2Cl2]（1），并通过红外光谱、电喷雾质谱、元素分析及单晶X射线衍射分析等方法对配合物1进行结构表征。配合物1为双核结构，中心铜（Ⅱ）离子为五配位扭曲四方锥几何构型，每个铜离子与1个Cl-以及来自配体（L-）的2个N原子和2个羟基O原子配位，2个铜（Ⅱ）离子通过羟基的桥联形成双核结构。MTT实验结果表明：配合物1对所选5种癌细胞株表现出不同的抑制活性，尤其是对MGC80-3细胞的抑制作用最明显，抑制率高达（84.30±1.28）%，其IC50值为（3.36±0.43）μmol·L-1。流式细胞术分析结果显示：配合物1能诱导MGC80-3细胞凋亡。在配合物1浓度为4.5 μmol·L-1时，MGC80-3的凋亡百分数为41.4%。此外，配合物1将MGC80-3细胞阻滞于G1期，从而抑制肿瘤细胞的生长。 | |
| 关键词: 三联吡啶 铜(Ⅱ)配合物 抗肿瘤活性 细胞周期 细胞凋亡 | |
| 基金项目: 国家自然科学基金（No.21431001）和教育部创新团队项目（No.IRT_16R15）资助 | |
| Abstract: A new copper(Ⅱ) complex [Cu2(μ-L-κO,O)2Cl2] (1) with 4-(2-hydroxy-3-chloro)phenyl-2,2':6',2'-tribi-pyridine(HL) as ligand was synthesized and characterized by elemental analysis, NMR, ESI-MS as well as single crystal X-ray diffraction analysis. Complex 1 is a binuclear structure, and the central copper(Ⅱ) is coordinated by one Cl- anion, two N atoms and two hydroxyl O atoms from L- to form five-coordination distorted square-pyramid geometric configurations; two copper centers are bridged by two hydroxyl O atoms. MTT assay was employed to test the inhibitory activities of complex 1 against five selected cancer cell lines. The results showed that inhibition rate of MGC80-3 cells was the highest, reaching(84.30±1.28)%, and its IC50 value was(3.36±0.43) μmol·L-1. Flow cytometry analytical results indicated that complex 1 could induce apoptosis of MGC80-3 cells. Under the complex 1 concentration of 4.5 μmol·L-1, the MGC80-3 cell apoptosis percentage was 42.2%. Complex 1 arrested MGC80-3 cells in G1 phase, thereby inhibiting the growth of tumor cells. | |
| Keywords: tribipyridine copper(Ⅱ) complex antitumor activity cell apoptosis cell cycle | |
| 投稿时间：2019-09-15 修订日期：2019-10-17 | |
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| 钟玉君,陈振锋,梁宏.4-(2-羟基-3-氯)苯基-2,2':6',2"-三联吡啶Cu(Ⅱ)配合物的合成、结构表征及抗肿瘤活性[J].无机化学学报,2019,35(11):2089-2094. | |
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