| 喹啉类单核锰(Ⅱ)和钴(Ⅱ)配合物的合成、结构、DNA/BSA键合及DNA切割活性 |
| Syntheses, Structures, DNA/BSA Binding and DNA Cleavage of Mononuclear Manganese(Ⅱ) and Cobalt(Ⅱ) Complexes with N,O-Chelating Quinoline Derivative Ligand |
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| 摘要: 合成了2个结构类似的喹啉类单核锰和钴配合物[ML（H2O）3]·H2O，其中M为Mn（1）、Co（2），Na2L为8-（羧基钠甲氧基）喹啉-2-甲酸钠。运用红外光谱、元素分析和X射线单晶衍射表征了其结构。利用电子吸收和发射光谱法研究了配合物与CT-DNA及BSA的键合作用及配合物对DNA的切割作用。晶体解析结果表明2个配合物为同构结构，配合物中心均为七配位的畸变五角双锥结构。钴配合物2与CT-DNA的键合能力强于锰配合物1，两者与BSA的作用机理为静态淬灭机理，而键合常数值大小为1>2。在以H2O2作为诱导剂时，在同等条件下，2切割DNA的能力明显增强。通过加入自由基捕获剂证明了配合物对DNA的切割机理为氧化切割机理，其中活性氧为OH·。 |
| 关键词: 配合物 喹啉类配体 DNA/BSA键合 DNA切割 |
| 基金项目: 山西农业大学引进人才科研启动金（No.2013YJ40，2013YJ41）、山西农业大学科技创新基金（No.2014013，2014005）、山西农业大学大学生科技创新项目（No.13-017，2015085）、山西省高等学校大学生创新创业训练项目（No.2015085，2015106）和山西省煤基重点项目（No.FT>201402-01）资助。 |
| Abstract: Two new mononuclear complexes[ML(H2O)3]·H2O (M=Mn(1) and Co(2)) of quinoline derivative ligand (Na2L=sodium 8-(carboxylatomethoxy)quinoline-2-carboxylate) have been synthesized and characterized. The complexes are isostructural and both metal centers are heptacoordinated with O6N donor sets and the geometry around metal centers can be best described as distorted pentagonal bipyramidal. Interactions of the complexes with CT-DNA and BSA have been explored by absorption and emission spectral methods. Binding abilities of the complexes to CT-DNA display a relative order:2>1, while the quenching mechanisms of BSA by both complexes are static procedures and the binding constant values follow the order:1>2. In the presence of H2O2 as a revulsant or an activator, compared with complex 1, the DNA cleavage efficiency of 2 exhibited more remarkable increases at the same conditions. Oxidative mechanism has been demonstrated by adding standard radical scavengers and the reactive oxygen species (ROS) responsible for the DNA cleavage is likely hydroxyl radicals (OH·). |
| Keywords: complexes quinoline ligand DNA/BSA binding DNA cleavage |
| 投稿时间：2016-06-15 修订日期：2016-09-29 |
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| 张永坡,杨佳佳,吕佳苑,高春艳,赵晋忠.喹啉类单核锰(Ⅱ)和钴(Ⅱ)配合物的合成、结构、DNA/BSA键合及DNA切割活性[J].无机化学学报,2016,32(12):2172-2182. |
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