| 钌(Ⅱ)配合物[Ru(dpq)2L]4+的合成、晶体结构及与G-四链体DNA的相互作用 | |
| Synthesis, Crystal Structure and Interactions with G-Quadruplex Structures of [Ru(dpq)2L]4+ | |
| 摘要: 以cis-[Ru(dpq)2Cl2]·2H2O(dpq=二吡啶[3,2-d:2',3'-f]二氮萘)为原料与5,5'-二(1-(三乙胺)甲基)-2,2'-联吡啶阳离子(L)合成钌(Ⅱ)配合物[Ru(dpq)2L](PF6)4,并研究了该配合物与G-四链体DNA的作用:FRET实验表明,配合物对人端粒DNAh-telo具有选择性,其作用能力要强于同癌基因启动子区域的四链DNA,如c-myc和bcl2;CD光谱表明,在Na+和K+都不存在的情况下,配合物能诱导h-telo形成平行结构;此外,紫外和发射光谱都显示,配合物在K+溶液中与h-telo的作用力要大于在Na+溶液中的。 | |
| 关键词: 钌(Ⅱ)配合物 G-四链体 选择性 | |
| 基金项目: 国家自然科学基金(No.21101034),广东省优秀青年教师培养计划项目(No.Yq2013086,Yq2013151),东莞市科技计划项目(No.2011108102046),广东省大学生创新课题(No.1057112037)项目,2014年广东省本科高校教学质量与教学改革工程立项建设项目资助。 | |
| Abstract: Based on cis-[Ru(dpq)2Cl2]·2H2O (dpq=dipyrido[3,2-d:2',3'-f]quinoxaline) and 5,5'-di(1-(triethylamm-onio)methyl)-2,2'-dipyridyl cation ligand (L), the complex [Ru(dpq)2L](PF6)4 was synthesized and structurally characterized. The interactions of the complex with different G-quadruplexes were investigated. FRET melting assay proved that the complex bonds more strongly to h-telo than to promoters, such as c-myc and bcl2. CD studies show that Ru(Ⅱ) complex can induce the formation of parallel G-quadruplex of h-telo in the absence of Na+ or K+. Results of absorption and emission titration indicated that the complex has a higher DNA affinity with h-telo in K+ buffer than in Na+ buffer. | |
| Keywords: Ru(Ⅱ) complex G-quadruplex DNA selectivity | |
| 投稿时间：2014-11-28 修订日期：2015-03-22 | |
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| 孙静,宋兴栋,陈文秀,赵轩昊,陈嘉曦,贾振斌,郝洪庆.钌(Ⅱ)配合物[Ru(dpq)2L]4+的合成、晶体结构及与G-四链体DNA的相互作用[J].无机化学学报,2015,31(5):865-872. | |
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