| 5-氨基间苯二甲酸敏化LaF3：Tb发光纳米粒子的合成及其细胞成像 |
| LaF3∶Tb Nanoparticles Coated with 5-Aminoisophthalic Acid Sensitizer and Their Applications in Immunolabeling and Imaging of Hela Cells |
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| 摘要: 以稀土硝酸盐、NH4F、5-氨基间苯二甲酸（AIPA）和柠檬酸为反应原料，水热法一锅合成了水溶性良好的AIPA敏化LaF3：Tb（AIPA-LaF3：Tb）纳米粒子。采用X射线衍射、透射电镜、红外光谱等对粒子进行了表征。结果表明，合成粒子为六方晶系的LaF3晶体，粒径为8 nm左右，粒子表面包覆了AIPA。测试了合成粒子的发光性能，其最大激发和发射波长分别为348和547 nm，粒子中存在着AIPA向Tb3+的能量传递。和LaF3：Ce，Tb粒子相比，AIPA-LaF3：Tb粒子的激发波长红移了94 nm，发光强度增大。在合成的基础上，对AIPA-LaF3：Tb粒子进行了氨基化修饰，然后将氨基化粒子和兔抗人CEA抗体偶联，最后用偶联了抗体的AIPA-LaF3：Tb粒子对HeLa细胞进行免疫标记与成像。 |
| 关键词: LaF3：Tb 5-氨基间苯二甲酸 发光纳米粒子 细胞成像 |
| 基金项目: 国家自然科学基金（No.21205139）资助项目。 |
| Abstract: 5-aminoisophthalic acid (AIPA) sentisized LaF3:Tb nanoparticles (AIPA-LaF3:Tb) with good water solubility were synthesized by one-step hydrothermal method using Ln(NO3)3(Ln=La/Tb), NH4F, AIPA and citrate acid as raw materials. The particles were characterized by XRD, TEM and FTIR. The results show that the as-prepared particles are single-phase LaF3 crystals coated with AIPA in hexagonal system with narrowly distributed particles size of about 8 nm. The luminescence properties of the particles were investigated and the energy transfer between AIPA and Tb3+ was observed with the strongest excitation peak of 348 nm and the strongest emission peak of 547 nm. Compared with those of LaF3:Ce,Tb nanoparticles, the excitation wavelength of AIPA-LaF3:Tb nanoparticles has a red-shift of 94 nm and the emission intensity is increased markedly.The results for lifetime evaluation of AIPA-LaF3:Tb nanoparticles reveal that the luminescence of AIPA-LaF3:Tb nanoparticles originates from the Tb3+ near or at the surfaces of the particles. After preparation, AIPA-LaF3:Tb nanoparticles were coated with amino-SiO2, and then were covalently linked to rabbit anti-CEA polyclone antibodies to form the conjugates. Finally, based on the specific combination of carcinoembryonic antigen (CEA) expressed on the surface of Hela cells with anti-CEA antibodies, AIPA-LaF3:Tb nanoparticles were used in immunolabeling and imaging of Hela cells. |
| Keywords: LaF3:Tb 5-aminoisophthalic acid luminescent nanoparticle cell imaging |
| 投稿时间：2014-04-15 修订日期：2014-06-04 |
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| 张永芳,于永丽,张宁,王猛,徐淑坤.5-氨基间苯二甲酸敏化LaF3：Tb发光纳米粒子的合成及其细胞成像[J].无机化学学报,2014,30(10):2315-2322. |
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