| CoFe2O4纳米晶与牛血清白蛋白和牛血红蛋白的相互作用：吸附、纳米晶的团聚及蛋白构象的变化 | |
| Interactions of CoFe2O4 Nanocrystals with Bovine Serum Albumin and Hemoglobin：Adsorption, Aggregation and Protein Conformational Changes | |
| 摘要: 水热制备了约10 nm的CoFe2O4纳米晶，通过Zeta电势、动态光散射（Dynamic Light Scattering，DLS）和傅立叶变换红外光谱（FTIR）技术研究了纳米晶与牛血清白蛋白（Bovine Serum Albumin，BSA）和牛血红蛋白（Hemoglobin）的相互作用。纳米晶对BSA和血红蛋白都有很强的吸附，其中对血红蛋白的吸附符合静电吸附的规律，而对BSA的吸附则不符合静电吸附的规律。在pH=5.5和7.0时纳米晶对BSA和血红蛋白的吸附容量分别达到237.9 mg·g-1和256.9 mg·g-1。DLS结果表明蛋白质能够导致纳米晶团聚。吸附BSA或血红蛋白后，纳米晶的DLS粒径由51 nm分别增大到472 nm和114 nm。CoFe2O4纳米晶还导致了蛋白质FTIR谱发生明显变化。BSA和血红蛋白的酰胺I带由于纳米晶的作用分别减少了4 cm-1和6 cm-1。 | |
| 关键词: CoFe2O4纳米晶 BSA 牛血红蛋白 相互作用 | |
| 基金项目: 国家自然科学基金（No.51172085）资助项目。 | |
| Abstract: Cobalt ferrite (CoFe2O4) nanocrystals (approximately 10 nm) were prepared by hydrothermal method and their interactions with Bovine Serum Albumin (BSA) and bovine hemoglobin were investigated by Zeta potential, Dynamic Light Scattering (DLS) and FTIR Spectroscopy techniques. Results show that nanocrystal-hemoglobin adsorption correlates to electrostatic attractive/repulsive interaction, whereas BSA does not follow this scheme. The corresponding adsorption capacity of BSA and hemoglobin reaches a maximum value of 237.9 mg·g-1 and 256.9 mg·g-1 at pH value of 5.5 and 7.0, respectively. DLS measurements indicate that protein adsorption have led to nanocrystal-protein aggregates formation. Comparing to the hydrodynamic size of bare nanocrystals (51 nm), that for BSA and hemoglobin increases to 472 nm and 114 nm respectively upon protein adsorption. The interaction with nanocrystal also induces protein conformation changes. The amide I band in IR spectrum shifts 4 cm-1 and 6 cm-1, respectively, to lower wavenumbers in the case of BSA and hemoglobin. | |
| Keywords: CoFe2O4 nanocrystal BSA hemoglobin interaction | |
| 投稿时间：2013-12-28 修订日期：2014-03-26 | |
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| 李强,姬晓旭,张凯,黄新堂,熊丽.CoFe2O4纳米晶与牛血清白蛋白和牛血红蛋白的相互作用：吸附、纳米晶的团聚及蛋白构象的变化[J].无机化学学报,2014,30(8):1961-1968. | |
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