| 三种钴乙醇酸配合物与牛血清白蛋白相互作用的荧光分析 | |
| Interaction Between Three Cobalt(Ⅱ) Glycollates and Bovine Serum Albumin Studied by Fluorescence Spectrum | |
| 摘要: 利用溶液法合成了3种钴乙醇酸配合物，并对配合物cis-[Co2（Hgly）4（2，2’-bpy）2]（Hgly=glycolic acid，2，2’-bpy=2，2’-bipyridine）（3）进行了X-射线单晶衍射表征，结果表明：该配合物为三斜晶系，空间群为P21/c，a=1.003 7（3）nm，b=2.022 6（5）nm，c=1.660 1（4）nm，β=119.942（13）°，V=2.920 3（13）nm3.利用荧光光谱法分别研究了室温下这3种配合物与BSA相互作用的结果，并测定了不同温度下配合物2和3与牛血清白蛋白相互作用的荧光强度的变化，确定了配合物2和3对牛血清白蛋白的荧光淬灭方式均为静态淬灭；同时分析了这两种配合物与牛血清白蛋白相互作用时的结合常数、结合位点数以及热力学函数与温度变化之间的关系，进一步讨论了这两种配合物分别与BSA相互作用时的作用力类型。综合以上结果表明：当分别含有邻菲咯啉和2，2’-联吡啶配体的配合物与BSA相混合时，它们都能与BSA相互作用，且主要的作用力都是氢键和范德华力，但它们对蛋白质中色氨酸的构象或其周围环境的影响却是不同的。 | |
| 关键词: 钴离子 乙醇酸 牛血清白蛋白 荧光光谱 | |
| 基金项目: 国家自然科学基金资助项目（No.21275127），福建省教育厅基金（No.JA12216）. | |
| Abstract: Three neutral complexes of cobalt(Ⅱ) glycollates had been synthesized in the solution, in which cis-[Co2(Hgly)4(2,2'-bpy)2] (3) (Hgly=glycolic acid, 2, 2'-bpy=2,2'-bipyridine) was characterized by X-ray structural analysis. It crystallized in the triclinic system, space group P21/c, a=1.003 7(3) nm, b=2.022 6(5) nm, c=1.660 1(4) nm, β=119.942(13)°,V=2.920 3 (13) nm3. The interaction between bovine serum albumin (BSA) and complexes 1~3 had been investigated with fluorescence spectra at room temperature respectively. The fluorescence quenching was shown as static quenching for 2 and 3 based on fluorescence spectra at the different temperatures. The binding constants, binding sites and thermodynamic functions were calculated. The interaction sites and binding force of BSA with complexes 2 and 3 were proposed. These results showed that when the complexes containing phenanthroline and 2,2'-bipyridine interacted with BSA respectively, the ligands of 1,10-phenanthroline and the 2,2'-bipyridine both perturbed the microenvironment close to tryptophan residues of BSA, but the effects were different. | |
| Keywords: cobalt(Ⅱ) glycolic acid BSA fluorescence spectrum | |
| 投稿时间：2013-12-01 修订日期：2013-12-25 | |
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| 林海彬,周朝晖,王庆华,汪庆祥.三种钴乙醇酸配合物与牛血清白蛋白相互作用的荧光分析[J].无机化学学报,2014,30(7):1559-1566. | |
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