| 金属元素组与血清白蛋白的竞争结合反应性能研究 |
| Study on Competing Reaction of Metallogroup Binding with Serum Albumin |
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| 摘要: 利用亲和毛细管电泳（Affinity capillary electrophoresis，ACE）法研究金属元素组和血清白蛋白（Bovine serum albumin，BSA）的竞争结合反应性能。基于位点结合模型，构建双金属组[Zn2+，Cu2+]与血清白蛋白结合反应模型，建立多元金属组与生物大分子竞争结合的理论方程，测定结合参数并解析动力学机制。结果表明，金属元素组[Zn2+，Cu2+]与BSA发生竞争结合反应形成配合物Zn2+-BSA和Cu2+-BSA.依据有效淌度变化，通过建立的理论方程非线性拟合竞争结合反应的平均表观结合常数KZn2+-BSA=4.01×104 L·mol-1、KCu2+-BSA=7.75×104 L·mol-1.结合反应均为快平衡反应，Cu2+对Zn2+离子的结合作用有明显拮抗作用。分析ACE谱显示配合物的峰高与配体结合能力大小、配合物稳定性之间存在量效关系。 |
| 关键词: 金属元素组 表观结合常数 配合物 量效关系 |
| 基金项目: 国家自然科学基金资助项目（No.20877072），浙江科技厅公益资助项目（No.2012C37055）. |
| Abstract: The performance of competing binding reactions between metallogroup and bovine serum albumin was investigated using affinity capillary electrophoresis(ACE) method. Based on the site binding model, a model about the interaction between [Zn2+, Cu2+] as the ligand and BSA as the receptor was established, a theoretical equation of competing binding reaction between multimetal-ions and macromolecule has been suggested, the binding parameters and dynamic mechanism were also measured and analyzed. The result show that, the competitive combined reactions of metallogroup [Zn2+, Cu2+] with BSA were reacted to form compounds Zn2+-BSA and Cu2+-BSA. Based on the changes of the effective mobility, the mean apparent competition binding constant was determined through established theoretical equation by using non-linear fitting method, and the average apparent binding constant KZn2+-BSA=4.01×104 L·mol-1, KCu2+-BSA=7.75×104 L·mol-1. The binding reactions of Zn2+/Cu2+-BSA are fast equilibrium reactions, and that Cu2+ had antagonism effect on Zn2+. Dosage-dependent effect also exists among the peak height of compounds with the ligand strength of binding reactions and stability of compounds. |
| Keywords: metallogroup apparent binding constant complex compound dosage-dependent effect |
| 投稿时间：2013-11-24 修订日期：2014-03-30 |
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| 郭明,黄凤琴,李铭慧,刘敏.金属元素组与血清白蛋白的竞争结合反应性能研究[J].无机化学学报,2014,30(7):1549-1558. |
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