| 2, 4-二氨基-6-（2’-吡啶基）均三嗪铜（Ⅱ）配合物的结构、抗菌活性及DNA作用 |
| Structure, Antibacterial Activities and DNA Interaction of a Copper(Ⅱ) Complex Derived from 2,4-Diamino-6-(2’-pyridyl)-1,3,5-triazine |
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| 摘要: 通过2，4-二氨基-6-（2’-吡啶基）均三嗪和高氯酸铜（Ⅱ）反应合成了一个配合物：[Cu（H2O）（PyTA）2]（ClO4）2[PyTA=2，4-二氨基-6-（2’-吡啶基）均三嗪]。通过元素分析、红外光谱、紫外可见光谱、摩尔电导率和X-射线单晶衍射等方法对其进行了表征。结果表明该配合物晶体属单斜体，P21/c空间群，其晶体学参数：a=0.98024（6）nm；b=1.24831（7）nm，c=2.15727（11）nm；β=108.657（3）°；Z=4，V=2.5010（2）nm3，R1=0.0543，wR2=0.1506。另外，应用试管二倍稀释法测定了配合物的抗菌活性，通过电子吸收光谱、荧光光谱、粘度测定及琼脂糖凝胶电泳等方法研究了配合物与DNA的作用。结果表明，与2，4-二氨基-6-（2’-吡啶基）均三嗪相比较，该配合物具有良好的抗菌活性，以插入模式与CT-DNA作用，并且在抗坏血酸存在下通过羟基自由基·OH切割pBR322DNA。 |
| 关键词: 铜（Ⅱ）配合物 2, 4-二氨基-6-（2-吡啶基）均三嗪 晶体结构 抗菌活性 DNA |
| 基金项目: 华南农业大学211工程重点项目（No.2009B010100001）。 |
| Abstract: Acopper(Ⅱ) complex: [Cu(H2O)(PyTA)2](ClO4)2[PyTA=2,4-diamino-6-(2'-pyridyl)-1,3,5-triazine], was synthesized and characterized by elemental analysis, molar conductivity, IR, UV-Vis and single crystal X-ray diffraction. The crystal belongs to monoclinic, space group P21/c, with the crystal cell parameters: a=0.98024(6) nm, b=1.24831(7) nm, c=2.15727(11) nm; β=108.657(3)°; Z=4, V=2.5010(2) nm3, R1=0.0543, wR2=0.1506. The complex was assayed against gram-positive (S.aureus, B. subtilis, B. thuringiensis) and gram-negative (X. oryzae, Salmonella, E. coil)bacteria by doubling dilutions method, and the interaction of the complex to DNA was investigated by electronic absorption, fluorescence, viscosity measurement and agarose gel electrophoresis assay. The results showed that the complex has good antibacterial activities, and could bind to CT-DNA by an intercalative mode, and cleave pBR322 DNAby hydroxyl radicals as the active species in the presence of Vit C. |
| Keywords: copper(Ⅱ) complex 2,4-diamino-6-(2’-pyridyl)-1,3,5-triazine crystal structure antibacterial activity DNA |
| 投稿时间：2013-05-05 修订日期：2013-12-21 |
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| 区志镔,黄山华,傅夏兵,朱丽,乐学义.2, 4-二氨基-6-（2’-吡啶基）均三嗪铜（Ⅱ）配合物的结构、抗菌活性及DNA作用[J].无机化学学报,2014,30(5):1017-1024. |
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