| 重金属Cr(Ⅵ)与血清蛋白质组的结合反应性能研究 |
| Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome |
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| 摘要: 利用亲和毛细管电泳(Affinity capillary electrophoresis, ACE)方法,基于位点结合模型,构建配体(Cr(Ⅵ))-血清蛋白质组受体(Fetal bovine serum, FBS)结合反应方程,研究重金属离子Cr(Ⅵ)和血清蛋白质组的结合反应性能。结果表明,组学条件下,Cr(Ⅵ)与FBS中高丰度组分白蛋白(Bovine serum albumin, BSA)、转铁蛋白(Bovine apo transferrin, bATF)、免疫球蛋白(Bovine IgG, IgG)之间形成Cr(Ⅵ)-蛋白质络合物;结合反应均为快平衡反应;依据有效淌度的变化,通过结合方程测得结合反应的表观结合常数KCr(Ⅵ)-bATF=1.27×104 L·mol-1、KCr(Ⅵ)-IgG=4.91×104 L·mol-1、KCr(Ⅵ)-BSA=8.98×104 L·mol-1;同时根据实验数据非线性模拟结果表明,Cr(Ⅵ)与FBS中高丰度组分发生相互作用的强度与Cr(Ⅵ)浓度之间存在明显的量效关系。本文有关工作可为重金属离子-蛋白质络合物结合性能等生物无机化学研究内容提供参考。 |
| 关键词: Cr(Ⅵ) 血清蛋白质组 表观结合常数 络合物 量效关系 |
| 基金项目: 国家自然科学基金(No.20877072),浙江科技厅公益项目(No.2012C37055) 资助项目。 |
| Abstract: The binding reaction between heavy metal ions Cr(Ⅵ) and serum proteome was investigated using affinity capillary electrophoresis(ACE) method. Based on the site binding model, a model about the interaction between Cr(Ⅵ) as the ligand and FBS as the receptor, was established. The binding performance of heavy metal ions Cr(Ⅵ) and the serum proteome was studied under proteomic conditions, and the results showed that, Cr(Ⅵ)-protein complexes formed between Cr(Ⅵ) and the high abundance component albumin in FBS, such as albumin (bovine serum albumin, BSA), transferrin protein (Bovine apo transferrin, bATF), immunogolobulin (Bovine IgG, IgG) and so on, and the binding reactions of Cr(Ⅵ) -FBS are fast equilibrium reactions. The apparent binding constant was determined through non-linear fitting binding reaction and based on changes of the effective mobility, and KCr(Ⅵ)-bATF=1.27×104 L·mol-1, KCr(Ⅵ)-IgG=4.91×104 L·mol-1, KCr(Ⅵ)-BSA=8.98×104 L·mol-1. Dosage-dependent effect exists among the concentration of Cr(Ⅵ) and the strength of the binding reactions. This work has referential meaning for understanding deeply the binding reaction mechanism of chromium(Ⅵ) with serum proteome. |
| Keywords: Cr(Ⅵ) serum proteome apparent binding constant complex dosage-dependent effect |
| 投稿时间：2012-12-25 修订日期：2013-01-31 |
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| 郭明,黄凤琴,刘咪咪,李铭慧.重金属Cr(Ⅵ)与血清蛋白质组的结合反应性能研究[J].无机化学学报,2013,29(5):1037-1044. |
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