| 硅烷偶联剂制备及对磷灰石-硅灰石生物玻璃陶瓷的表面改性 | |
| Preparation of a Silane Coupling Agent and Surface Modification of Apatite-Wollastonite Bioactive Glass-Ceramic | |
| 摘要: 采用谷氨酸与硅烷偶联剂(KH-792)缩合反应,制备出一种含酰胺基的硅烷偶联剂,利用其与材料之间的化学结合,将谷氨酸共价固定至磷灰石-硅灰石生物玻璃陶瓷表面,实现表面改性。将改性后的磷灰石-硅灰石生物玻璃陶瓷与人骨肉瘤细胞MG63共培养,评价其体外细胞生物相容性。应用FTIR、SEM、XPS等测试技术分别对合成的偶联剂、改性后的陶瓷材料进行结构、表面形貌及细胞生长情况的表征,结果表明:谷氨酸与KH-792的反应产物有酰胺基生成;含酰胺基的硅烷偶联剂能对磷灰石-硅灰石生物玻璃陶瓷表面进行有效改性;共培养3 d后细胞在材料材料表面大量贴附,MTT实验也证明增殖良好,即含酰胺基的硅烷偶联剂能改善磷灰石-硅灰石生物玻璃陶瓷的体外细胞生物相容性。 | |
| 关键词: 硅烷偶联剂 谷氨酸 磷灰石-硅灰石生物玻璃陶瓷 表面改性 | |
| 基金项目: 四川大学2011年青年基金(No.2082604154120)资助项目。 | |
| Abstract: In this work, a kind of silane coupling agent was prepared by condensation reaction of the glutamic acid and silane coupling agent (KH-792). The Glutamic acid was attached to the surface of Apatite-wollastonite bioactive glass-ceramic (A-W GC) by the bifunctional cross-linking agent. To evaluate in vitro cytocompatibility, the surface modified material was co-cultured with the 5th generation human osteosarcoma cell MG63. Fourier Transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), and X-ray Photoelectron Spectroscopy (XPS) were used to character the structure and performance of the synthetic coupling agent and the surface modified A-W GC, respectively. The results show that the Glutamic acid and KH-792 were connected by amide groups, and A-W GC is effectively modified by the synthetic coupling agent. The MG63 cell experiments show that cells adhered to the surface of the material, and the cell proliferation on the surface of the material was promoted. It is an effective method to improve the cell compatibility of A-W GC by surface modification. | |
| Keywords: silane coupling agent glutamic acid apatite-wollastonite bioactive glass-ceramic surface modification | |
| 投稿时间：2012-10-19 修订日期：2013-01-07 | |
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| 龙沁,周大利,张翔,卫冬娟,柳淑婧,陈冬宁.硅烷偶联剂制备及对磷灰石-硅灰石生物玻璃陶瓷的表面改性[J].无机化学学报,2013,29(5):948-952. | |
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