| 二茂铁修饰Aβ疏水段五肽的电化学性质及自组装研究 |
| Electrochemical Properties and Self-assembly of Ferrocene Modified Hydrophobic Pentapeptide Fragment of β-Amyloid |
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| 摘要: 五肽Lys-Leu-Val-Phe-Phe(即KLVFF)是β-淀粉样肽(Aβ)疏水核心片段，其自组装方式对Aβ的聚集具有重要影响。在本文中，首先对二茂铁修饰的五肽，即Fc-Lys(Z)-Leu-Val-Phe-Phe-OMe(简称 Fc-KLVFF)的电化学性质进行了研究，结果表明，在0.4~0.8 V范围内出现了一对可逆的氧化还原峰，氧化峰和还原峰电位分别为0.626和0.544 V，峰电位之差ΔEp为82 mV, 峰电流密度之比Ipa/Ipc为1.08，扩散系数D0=1.784×10-6 cm2·s-1。其次通过TEM观察到Fc-KLVFF在甲醇溶液中自组装成纳米管，傅里叶红外光谱分析表明纳米管的二级结构主要为β-折叠，紫外-可见和荧光光谱证实苯丙氨酸残基芳香环之间的 π-π堆积作用在其自组装成纳米管时起着重要作用。最后提出了 Fc-KLVFF 的自组装机理模型。 |
| 关键词: 二茂铁 β-淀粉样肽 透射电镜 电化学 |
| 基金项目: |
| Abstract: The self-assembly of ferrocene modified hydrophobic pentapeptide fragment of β-amyloid, Fc-KLVFF, is studied. Fc-KLVFF can self-assemble into nanotubes in methanol, as confirmed by transmission electron microscopy. The secondary structure of the nanotubes is characterized by FT-IR, fluorescence spectroscopy and UV-Visible spectroscopy, which indicate the important role of aromatic π-π stacking interactions between phenylalanine residues in driving β-sheet self-assembly. The electrochemical results showed that the Fc-KLVFF exhibits a pair of well-defined redox waves at oxidation potential (Ea) of 0.626 V and reduction potential (Ec) of 0.544 V, respectively, and the ratio of Ipa/Ipc is 1.08. Finally, the model for the self-assembly of Fc-KLVFF is proposed. |
| Keywords: ferrocene beta-amyloid TEM electrochemistry |
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| 魏传晚,李 娟,刘又年.二茂铁修饰Aβ疏水段五肽的电化学性质及自组装研究[J].无机化学学报,2013,29(1):45-49. |
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