| 不对称大环双核Ni(Ⅱ)配合物的DNA切割性能研究(英文) | |
| Studies on an Unsymmetrical Macrocyclic Dinuclear Ni(Ⅱ) Complex Interaction with DNA | |
| 摘要: 本文利用模板导向的方式合成了一种含有不对称侧链的大环双核金属Ni(Ⅱ)配合物(大环配体由3-溴甲基-5-甲基水杨醛，乙二胺，N，N-二(氨丙基)-2呋喃甲胺分步合成得到)，其结构通过红外光谱、元素分析、X-射线单晶衍射进行了表征。利用紫外光谱、分子荧光和粘度试验对配合物与DNA的相互作用进行了研究，结果表明配合物与CT-DNA的结合常数K=4.8×104 mol-1·L荧光淬灭常数Ksv=7.12×103 mol-1·L；同时机理研究表明配合物与CT-DNA结合方式为插入模式。 | |
| 关键词: 同双核配合物 大环配合物 结合常数 粘度 DNA切割 | |
| 基金项目: | |
| Abstract: An unsymmetrical side chains macrocyclic dinuclear Ni(Ⅱ) complex has been obtained by template-directed synthesis (the macrocyclic ligand was synthesized by 3-bromomethyl-2-hydroxy-5-methylbenzaldehyde, ethane-1,2-diamine, and N1-(3-aminopropyl)-N1-(furan-2-ylmethyl)propane-1,3-diamine), the structure has been characterized by IR spectrum, elemental analysis and X-ray crystallography determination. The interactions of the complex with DNA have been measured by UV spectroscopy, fluorescence spectroscopy and viscosity experiments. Absorption spectroscopic investigation reveals of intercalative binding of the complex with DNA with a binding constant of 4.8×104 mol-1·L. Fluorescence spectroscopy shows that the complex displaces EB and bind to DNA with a quenching constant of 7.12×103 mol-1·L. The appearance of both increased CD bands nearing 371 nm gives evidence for effective complex-DNA binding. The agarose gel electrophoresis studies show that the complex displays effective DNA cleavage activity in the absence of any external agents. CCDC: 835716. | |
| Keywords: homodinuclear complex macrocyclic complex binding constant viscosity DNA cleavage | |
| 摘要点击次数: 1389 | |
| 全文下载次数: 1973 | |
| 丁 超,陈云峰,刘 明,宋会婷,周 红,潘志权.不对称大环双核Ni(Ⅱ)配合物的DNA切割性能研究(英文)[J].无机化学学报,2012,28(4):801-808. | |
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