| 混配氧钒配合物[VO(Naph-Phe)(Phen)]的合成、晶体结构及与DNA作用研究 |
| Synthesis, Crystal Structure and DNA-Binding Studies of an Oxovanadium(Ⅳ) Complex [VO(Naph-Phe)(Phen)] with Mixed-Ligands |
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| 摘要: 合成了一个新的L-苯丙氨酸萘酚醛希夫碱(Naph-Phe)和邻菲咯啉(Phen)混配氧钒配合物[VO(Naph-Phe)(Phen)]，通过元素分析和红外光谱进行了表征。X-射线单晶衍射分析表明，该晶体属于单斜晶系，P21/c空间群，晶胞参数为：a=1.027 85(11) nm，b=2.186 5(3) nm，c=1.171 150(13) nm，β=94.747 0(10)°，V=2.622 9(4) nm3，Z=4，F(000)=1 164，R1=0.076 7，wR2=0.165 5，S=1.092。利用紫外吸收光谱、荧光光谱、圆二色光谱和粘度测量等研究了配合物与小牛胸腺DNA(CT-DNA)的作用，结果表明配合物以插入方式与CT-DNA作用。琼脂糖凝胶电泳法研究了配合物与pBR322 DNA的作用，表明它可切割超螺旋型DNA为缺刻型DNA。 |
| 关键词: 钒配合物 希夫碱 晶体结构 DNA |
| 基金项目: |
| Abstract: A new oxovanadium complex with mixed-ligands of a Schiff base (Naph-Phe) derived from L-phenylal-anine and 2-hydroxy-1-naphthaldehyde and 1,10-phenanthroline (Phen), [VO(Naph-Phe)(Phen)], has been synthe-sized and characterized by elementary analysis, IR and single crystal X-ray diffraction. It was shown that the complex crystallized in monoclinic crystal system, P21/c space group with the cell parameters: a=1.027 85(11) nm, b=2.186 5(3) nm, c=1.171 150(13) nm, β=94.747 0(10)°, V=2.622 9(4) nm3, Z=4, F(000)=1 164, R1=0.076 7, wR2=0.165 5, S=1.092. The DNA-binding properties have been investigated by UV absorption, fluorescence, CD spectra and viscosity measurement. The results indicate that the complex binds to CT-DNA in an intercalative mode. Meanwhile, the cleavage reaction on plasmid DNA has been monitored by submarine gel electrophoresis. The complex could cleave circular plasmid pBR322 DNA to nicked form. CCDC: 770157. |
| Keywords: vanadium complex Schiff base crystal structure DNA |
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| 边 琳,李连之,王 霞,黄 蕾,蒲雪炜,董建方.混配氧钒配合物[VO(Naph-Phe)(Phen)]的合成、晶体结构及与DNA作用研究[J].无机化学学报,2011,27(4):649-654. |
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