| Fe3+和Fe2+对原代培养的成骨细胞增殖、分化和矿化功能的影响(英文) |
| Effects of Fe3+ and Fe2+ on Proliferation, Differentiation and Mineralization Function of Primary Osteoblasts in vitro |
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| 摘要: 采用噻唑蓝(MTT)法、碱性磷酸酶(ALP)比活性测定、油红O染色和茜素红染色及定量分析，研究了不同浓度的Fe3+和Fe2+对原代培养的成骨细胞增殖、分化及矿化功能的影响。结果表明：浓度为1×10-9~1×10-4 mol·L-1的Fe3+和Fe2+促进成骨细胞增殖，但是在较高浓度1×10-3 mol·L-1时，它们则抑制成骨细胞增殖。与成骨细胞作用48 h，浓度为1×10-8~1×10-4 mol·L3+的Fe3+和Fe2+抑制其分化，但在较低的浓度1×10-9 mol·L-1时则对其分化没有影响；进一步延长作用时间为72 h，Fe3+对成骨细胞分化没有影响，除1×10-6 mol·L-1浓度的Fe2+促进成骨细胞分化外，其他浓度的Fe2+则抑制其分化；测试浓度下的Fe3+对成骨细胞向脂肪细胞的横向分化表现为抑制或没有影响，而Fe2+的影响则依赖于浓度和作用时间。在1×10-8~1×10-5 mol·L-1浓度范围内，Fe3+和Fe2+对矿化结节的影响表现出相反的效应。在较高浓度(1×10-4 mol·L-1)下，它们促进矿化节结的形成，而在较低浓度(1×10-9 mol·L-1)下，Fe3+抑制矿化节结的形成，Fe2+则没有影响。结果提示：浓度，作用时间和铁离子的价态都是影响Fe3+和Fe2+生物效应(从毒性到活性，从损伤到保护，从上调到下调)转变的关键因素。 |
| 关键词: 铁离子 成骨细胞 增殖 分化 矿化 |
| 基金项目: |
| Abstract: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromid(MTT), alkaline phosphatase(ALP) activity, oil red O assays and alizarin red-S(ARS) stain were used to evaluate the effects of Fe3+ and Fe2+ on proliferation, differentiation and mineralization function of primary osteoblasts(OBs) in vitro. The results indicate that both Fe3+ and Fe2+(1×10-9~1×10-4 mol·L-1) promote the proliferation of OBs, but turn to inhibit at a higher concentration of 1×10-3 mol·L-1. Both Fe3+ and Fe2+(1×10-8~1×10-4 mol·L-1) inhibit differentiation of OBs, but have no effect on differentiation at a lower concentration of 1×10-9 mol·L-1 for 48 h. Whereas, Fe3+ shows no effect on differentiation of OBs, Fe2+ begins to promote differentiation of OBs at concentration of 1×10-6 mol·L-1, but Fe2+ inhibits differentiation of OBs at other concentrations by prolonging the incubation time. Fe3+ inhibits adipocytic trans-differentiation of OBs or has no effects on adipocytic trans-differentiation of OBs at tested concentrations. The promotion or inhibition effect of Fe2+ on adipocytic trans-differentiation of OBs depends on concentration and incubation time. Fe3+ and Fe2+(1×10-8~1×10-5 mol·L-1) inhibit and promote the formation of mineralized matrix nodules of OBs, respectively. Fe3+ and Fe2+ promote the formation of mineralized matrix nodules at a higher concentration of 1×10-4 mol·L-1, but Fe3+ inhibits the formation of mineralized matrix nodules, Fe2+ shows no effect at a lower concentration of 1×10-9 mol·L-1. The results suggest that concentration, culture time and valence state of iron ion are key factors for switching the biological effects of Fe3+ and Fe2+ from toxicity to activity, from damage to protection, or from down-regulation to up-regulation. |
| Keywords: iron ion osteoblasts proliferation differentiation mineralization |
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| 张金超,李亚平,刘翠莲,孙 静,赵燕燕.Fe3+和Fe2+对原代培养的成骨细胞增殖、分化和矿化功能的影响(英文)[J].无机化学学报,2009,25(10):1835-1841. |
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