| 钙离子对HRPC结构和电催化活性影响的研究 |
| Effect of Calcium Ion on Structure and Electrocatalysis of Horseradish Peroxidase Isoenzyme C |
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| 摘要: 通过比较辣根过氧化物酶同工酶C(HRPC)中Ca2+移除前后的HRPC的光谱和电化学特性，阐明了Ca2+的存在对HRPC的重要作用。移除Ca2+后HRPC的紫外-可见吸收光谱发生了变化，Soret带的最大特征吸收峰以及α带和β带特征吸收峰均发生不同程度的蓝移和峰强减弱，表明其血红素微环境及结构发生了变化。移除Ca2+后的HRPC也可在与聚L型赖氨酸共修饰的石墨电极上表现出对H2O2的催化响应，但是天然HRPC比去除Ca2+后的HRPC对H2O2表现出更低的检测限，更宽的检测范围，更高的灵敏度以及更好的稳定性。实验结果表明，Ca2+对HRPC血红素微环境的保持、催化活性和稳定性均有重要的作用。 |
| 关键词: 生物电化学 辣根过氧化物酶同工酶C 钙离子 |
| 基金项目: |
| Abstract: Effect of Ca2+ on the structural and electrocatalytic properties of horseradish peroxidase isoenzyme C (HRPC) was investigated. UV-Vis absorption spectra of HRPC are found to be changed after removal of calcium. The absorption peaks of Soret region and α, β band are all blue-shifted and decreased, which indicates that the heme microenvironment and structure of HRPC can be changed due to calcium removal. Calcium-depleted HRPC is also able to catalyze the reduction of H2O2; however, native HRPC has lower detection limit, wider detection range, higher sensitivity to H2O2, as well as better stability. These results indicate that Ca2+ plays critical role in maintaining the heme microenvironment, catalytic activity and stability of HRPC. |
| Keywords: bioelectrochemistry horseradish peroxidase isoenzyme C calcium ion |
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| 周楠迪,朱彤阳,黄吟曦,刘山莉.钙离子对HRPC结构和电催化活性影响的研究[J].无机化学学报,2008,24(7):1089-1093. |
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