| pH值和钙离子对尖吻蝮蛇抗血小板凝集素二级结构的影响(英文) |
| Effects of pH Value and Ca2+ Ion on the Secondary Structure of Agkisacutacin from Agkistrodon Acutus Venom |
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| 摘要: 尖吻蝮蛇毒中抗血小板凝集素是凝血因子IX/凝血因子X结合蛋白，它具有抗凝血和抑制血小板凝集双重活性。用红外光谱、拉曼光谱和CD谱研究了抗血小板凝集素的二级结构以及pH值和钙离子对其二级结构的影响。用CD谱测得，在水溶液中，抗血小板凝集素的主要骨架构象为β-折叠(26.3%)和α-螺旋(19.6%)结构。拉曼光谱显示，在粉末状态，其α-螺旋含量显著降低。CD谱还表明，抗血小板凝集素在pH值3.0～11.0范围内保持稳定的天然结构，钙离子诱导的抗血小板凝集素结构变化是可逆的，钙离子在稳定抗血小板凝集素的天然结构中起重要作用。 |
| 关键词: 抗血小板凝集素 二级结构 圆二色谱 拉曼光谱 红外光谱 |
| 基金项目: |
| Abstract: Agkisacutacin isolated from the venom of Agkistrodon acutus is a coagulation factor IX/coagulation factor X-binding protein with marked anticoagulant- and platelet-modulating activities. The effects of pH value and Ca2+ ion on the secondary structure of Agkisacutacin have been studied by FTIR, Raman and circular dichroism (CD) spectroscopy. The main backbone conformations of holo-Agkisacutacin are α-helix (19.6%) and β-sheet (26.3%) as determined by CD spectroscopy. According to the Raman measurements, the content of α-helix of holo-Agkisacutacin in solid powder is less than that in solution. The conformation of Agkisacutacin is stable in the pH range of 3.0～11.0 as shown by CD spectroscopy. Ca2+-induced conformation change of Agkisacutacin is reversible. Ca2+ ion plays an important role in the stabilization of the structure of Agkisacutacin. |
| Keywords: agkisacutacin secondary structure circular dichroism Raman Resonance FTIR |
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| 陈结霞,徐小龙,王守业,张立云,刘清亮.pH值和钙离子对尖吻蝮蛇抗血小板凝集素二级结构的影响(英文)[J].无机化学学报,2007,23(5):849-853. |
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