| pH值和金属离子对尖吻蝮蛇抗凝血因子Ⅱ二级结构的影响 |
| The Effects of pH and Metal Ions on the Secondary Structure of Anticogulation Factor Ⅱ Studed by CD Spectroscopy |
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| 摘要: 圆二色谱(CD谱)测量表明，尖吻蝮蛇抗凝血因子Ⅱ(ACF Ⅱ)与抗凝血因子Ⅰ(ACF Ⅰ)的二级结构十分相似，它们的主要骨架构象都为反平行β-折叠和α-螺旋结构。pH值对天然ACF Ⅱ的构象有一定的影响，pH值从3到10增大时，α-螺旋含量逐渐减少，反平行β-折叠含量逐渐增大。钙和其他二价金属离子对ACF Ⅱ的骨架结构没有明显影响。三价稀土离子使ACF Ⅱ的α-螺旋含量升高，同时降低其β-转角和β-折叠的含量。 |
| 关键词: 抗凝血因子Ⅱ 酸度(pH) 二级结构 圆二色谱 |
| 基金项目: |
| Abstract: Anticoagulation factors holo-ACF Ⅱ and holo-ACF I have similar secondary structures and their main backbone conformations are anti-parallel β-sheet and α-helix determined by CD spectra. The contents of anti-parallel β-sheet and α-helix are 26.2%, 13.7% for holo-ACF Ⅱ, respectively. When pH value increases, the content of α-helix of ACF Ⅱ decreases, while the contents of anti-parallel β-sheet increases. No apparent effects of Ca2+, Sr2+, Mg2+ and transition metal ions (Zn2+, Mn2+ and Co2+) on conformations of the backbone of ACF Ⅱ are observed. The substitution of lanthanide ions for Ca2+ in ACF Ⅱ increases the content of α-helix, but decreases the content of β-sheet. |
| Keywords: anticoagulation factor Ⅱ pH secondary structure CD spectra |
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| 陈结霞,徐小龙,刘祥虎,刘清亮.pH值和金属离子对尖吻蝮蛇抗凝血因子Ⅱ二级结构的影响[J].无机化学学报,2006,22(1):69-72. |
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