| 牛血清白蛋白与两种金属配合物的作用研究 |
| Interaction of Bovine Serum Albumin with Twain Metal Complexes |
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| 摘要: |
| 关键词: UV-Vis光谱法 甲基百里酚蓝-Zn(Ⅱ)-配合物 牛血清白蛋白 茜素红S-Al(Ⅲ)-配合物 |
| 基金项目: |
| Abstract: The interaction of Methylthymol Blue(MTB)-Zinc(Ⅱ) compound and Alizarin Red S(ARS)-Aluminum(Ⅲ) compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn(Ⅱ)-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn(Ⅱ) complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn(Ⅱ) with BSA was ε=2.20×104 L·mol-1·cm-1. Conditional proportion were defined, nMTB∶nZn(Ⅱ)∶nBSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, nARS∶nAl(Ⅲ)∶nBSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al(Ⅲ) with BSA was K=8.80×108. The apparent molar absorptivity of ε=2.65×104 L·mol-1·cm-1. It is suggested that combination between BSA and MTB-Zn(Ⅲ) is due to coordination force. That combination between BSA and ARS-Al(Ⅲ) is due to the coordination bond and electrostatic force. |
| Keywords: UV-Vis spectroscopy methylthymol blue-zinc(Ⅱ) complex bovine serum albumin alizarin red S-aluminum(Ⅲ) complex |
| 投稿时间：2005-03-28 修订日期：2005-05-23 |
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| 刘海萍,王兴明,戴亚堂,宋绵新.牛血清白蛋白与两种金属配合物的作用研究[J].无机化学学报,2005,21(9):1422-1426. |
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