| Mg2+与胆汁蛋白质相互作用研究 |
| Study on the Interaction between Mg2+ and Biliary Protein |
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| 摘要: |
| 关键词: Mg2+ 胆汁蛋白质 荧光光谱 紫外可见光谱 |
| 基金项目: |
| Abstract: Biliary protein was isolated and purified by ammonium sulfate fractionation and Sephadex G-75 gel filtration column chromatography. FTIR showed that was a glycoprotein. Its molecular weight was determined as 34.7 kD by MS. The interaction between Mg2+ and biliary protein was studied by fluorescence spectroscopy and UV-Visible spectroscopy. The result showed the quenching mechanism of the combination of Mg2+ with biliary protein was a static quenching procedure. The protein underwent conformation changing and unfolding, more hydrophobic residues would be exposed to solvents after the Mg2+ was added. |
| Keywords: Mg2+ biliary protein fluorescence spectroscopy UV-Visible spectroscopy |
| 投稿时间：2004-09-01 修订日期：2004-10-27 |
| 摘要点击次数: 1287 |
| 全文下载次数: 1987 |
| 谢安建,姚成立,沈玉华.Mg2+与胆汁蛋白质相互作用研究[J].无机化学学报,2005,21(3):413-416. |
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