| 锌试剂-Cu(Ⅱ)金属配合物与牛血清蛋白作用研究 |
| A Study on the Reaction of Zincon-Cu(Ⅱ) Metal Complexes with Bovine Serum Albumin |
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| 摘要: |
| 关键词: 锌试剂-Cu(Ⅱ)配合物 牛血清蛋白(BSA) UV-Vis光谱 锌试剂-Cu(Ⅱ)-牛血清蛋白缔合物 |
| 基金项目: |
| Abstract: The interaction of Zincon(ZCN)-Cu(Ⅱ) metal complexe with bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution (pH=4.25). ZCN-Cu(Ⅱ)-BSA was a blueness color coordination compound, with maximum absorption at 605 nm with 10 nm, 60 nm and 140 nm of red shift compared to the ZCN-Cu(Ⅱ) complex, ZCN-BSA complex and ZCN. According to molar ratio method and dual wavelength method, the apparent molar absorptivity of ε was equal to 4.82×105 L·mol-1·cm-1. Conditional constants were defined as ZCN∶Cu(Ⅱ)∶BSA=10∶5∶2. Condition combination constant of ZCN-Cu(Ⅱ) and BSA K was 8.82×104. A combined mechanism was suggested between BSA and ZCN-Cu(Ⅱ) by electrostatic force (Primary action force) and distant water force. Position of Impact might be arginine, proline, histidine, tyrosine and cystine. Contemporary formation of electron transfer complexes is an important cause in forming ZCN-Cu(Ⅱ)-BSA complexes. |
| Keywords: ZCN-Cu(Ⅱ) coordination compound bovine serum albumin(BSA) UV-Vis spectra ZCN-Cu(Ⅱ)-bovine serum albumin coordination compound |
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| 王兴明,董发勤,丁立生,彭汝芳,廖辉伟.锌试剂-Cu(Ⅱ)金属配合物与牛血清蛋白作用研究[J].无机化学学报,2004,20(8):920-924. |
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