| 肌红蛋白在硅基表面自组装的研究 |
| The Self-Assembly Study of Myoglobin on Silicon Surface |
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| 摘要: |
| 关键词: 硅基 自组装单层膜 光电子能谱 原子力显微镜 肌红蛋白 |
| 基金项目: |
| Abstract: With the rapid development of bioanalytical methods and biosensors, it is desirable to immobilize a Self-assembled Monolayers with pure protein on inorganic surface. In this work, we tried to immobilize myoglobin on a surface of a silicon substrate as a model. At first, the cleaned silicon substrate was filled with 5% 3-aminopropyltrimethoxysilane (3-APTS). Then the formed SAMs were treated with glutaraldehyde and solution of myoglobin. Subsequently, the composition, structure and morphology of the thin film surface have been characterized by X-ray photoelectron spectroscopy (XPS) and Atomic force microscopy (AFM). The results indicate that myoglobin had been successfully immobilized on P100 Si substrate. This method will be especially useful for further protein biomolecule immobilization and advanced single molecule detection studies. |
| Keywords: silicon substrate SAM XPS AFM myoglobin |
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| 陈 浩,刘小鹏,陈慧兰.肌红蛋白在硅基表面自组装的研究[J].无机化学学报,2004,20(4):451-454. |
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