| 细胞色素ｃ氧化酶ＣｕＡ结构域蛋白的荧光猝灭研究 |
| Study on the Fluorescence Quenching of CuA Domain of Cytochrome c oxidase by KI |
|
| 摘要: |
| 关键词: 细胞色素ｃ氧化酶 ＣｕＡ结构域蛋白 荧光猝灭 |
| 基金项目: |
| Abstract: The fluorescence quenching spectra of the holo-CuA and apo-CuA domain of cytochrome c oxidase from Paracoccus versutus by KI were investigated.The results indicated that in solution part of the fluorophore tryptophanes of the CuA domain are exposed to the surface of the protein,and others buried inside of the hydrophobic core.The fluorescence quenching constants of holo-CuA and apo-CuA domain protein by KI are 1.39 and 2.24,respectively,which suggested that the bi-nuclear copper center [CuA+1.5-CuA+1.5] also plays a role on the structural stability of the protein. |
| Keywords: cytochrome c oxidase CuA domain fluorescence quenching |
| |
| 摘要点击次数: 1509 |
| 全文下载次数: 1582 |
| 李连之,宋爱新,黄仲贤.细胞色素ｃ氧化酶ＣｕＡ结构域蛋白的荧光猝灭研究[J].无机化学学报,2004,20(1):48-52. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| Support information: |
|
|
|
|
|
|
