| 镧离子对植物体内过氧化物酶活性的影响 | |
| Effect of Lanthanum Ion on Peroxidase Activity in Plant | |
| 摘要: 本文首次比较了在植物体内La3+与过氧化物酶(POD)的相互作用和在植物体外La3+与辣根过氧化物酶(HRP)的相互作用。在植物体内的研究表明,La3+能够明显改变POD的活性,并呈现低促高抑的“Hormesis效应”。用圆二色谱(CD)、紫外-可见(UV-Vis)吸收光谱和傅立叶变换红外(FTIR)光谱研究在植物体外La3+与HRP相互作用的结果表明,在模拟生理条件的溶液中,La3+对HRP的活性同样呈现低促高抑的＂Hormesis 效应＂。其作用机理可能是La3+通过与HRP中酰胺基的O或N键合,导致HRP二级结构变化,进而改变活性中心血红素中铁卟啉的化学结构,最终改变HRP的催化活性。 | |
| 关键词: 镧离子 过氧化物酶 辣根过氧化物酶 活性 | |
| 基金项目: | |
| Abstract: It is reported for the first time that the interaction between La3+ and POD in the plant was compared with that the interaction between La3+ and HRP in the mimic physiological solution. It was found that in the plant, La3+ could obviously change the activity of POD and show the “Hormesis effect”. The results of the investigation of the interaction between La3+ and HRP using CD spectroscopy, UV-Vis absorption spectroscopy and FTIR spectroscopy indicated that the interaction between La3+ and HRP in the mimic physiological solution also showed the “Hormesis effect”. It was found that La3+ could bond to O or N group of the amides in the polypeptide chains of HRP and thus cause the change in the secondary structure of HRP. It would change in the structure of the porphyrin group in the heme group of HRP and lead the change in the catalytic activity of HRP. | |
| Keywords: lanthanum ion peroxidases horseradish peroxidase activity | |
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| 张晓春,李 邨,陆天虹,丁晓岚,周 青,黄晓华.镧离子对植物体内过氧化物酶活性的影响[J].无机化学学报,2003,19(12):1285-1289. | |
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