| 核酸酶P1活性中心金属离子与氯化钴(Ⅱ)相互作用 |
| Study on the Interaction of Metal Ions in Active Center of Nuclease P1 with CoCl2 |
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| 摘要: 核酸酶P1是一种重要的DNA与RNA水解酶。本文利用ICP、VIS、NMR、酶活性测定等分析方法，首次拓展研究了核酸酶P1与CoCl2的直接相互作用。结果发现：核酸酶P1活性中心Zn(Ⅱ)离子可被外加CoCl2中的Co(Ⅱ)部分取代，而Co(Ⅱ)进入酶的活性中心，生成相应的酶衍生物“Co(Ⅱ)-P1”，并影响了酶的催化活性。与此同时还获得了Co(Ⅱ)进入核酸酶P1活性中心Zn2位上的酶衍生物 1H NMR谱。 |
| 关键词: 核酸酶P1 CoCl2 NMR 相互作用 |
| 基金项目: |
| Abstract: The paper using various ways， such as ICP， VIS， NMR and measurement of enzyme activity， extensively study the direct interaction of Nuclease P1 with inorganic metal compound (CoCl2). It has been found that in aqueous solution， there exists a direct interaction between the metal ions of the active center in the metalloenzyme (Nuclease P1) and external added CoCl2. As a result， part of the metal ions in metalloenzyme were substituted and the cor-responding metalloenzyme derivatives were produced and the catalytic activity of enzyme were changed， and the 1H NMR spectrum of Co(Ⅱ)-substituted nuclease P1 derivative of Co(Ⅱ) in Zn2 site of active center was firstly obtained. |
| Keywords: nuclease P1 CoCl2 NMR interaction |
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| 郑学仿,迟乃玉,张庆芳,袁玉莲,李晓艳,胡皆汉.核酸酶P1活性中心金属离子与氯化钴(Ⅱ)相互作用[J].无机化学学报,2003,19(4):377-380. |
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