| Ru(Ⅲ)、Rh(Ⅲ)、Pd(Ⅱ)离子与ct-DNA的相互作用研究 |
| Studies on Interactions of Ru(Ⅲ)，Rh(Ⅲ)，Pd(Ⅱ)with ct-DNA |
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| 摘要: 本文以中药小檗碱作为分子探针，在0.01mol·L-1醋酸-醋酸钠缓冲体系中，用紫外-可见吸收及荧光光谱法研究了Ru(Ⅲ)、Rh(Ⅲ)、Pd(Ⅱ)三种贵金属离子与DNA的键合相互作用。实验发现Ru(Ⅲ)离子对小檗碱-DNA二元体系的荧光有较强的猝灭作用;而Rh(Ⅲ)、Pd(Ⅱ)两种离子则对该二元体系产生显著的荧光敏化作用。考察了EDTA对贵金属离子、小檗碱及DNA三元混合体系的荧光光谱的影响，初步探讨了贵金属离子与DNA可能的键合机理。 |
| 关键词: 荧光探针 Ru(Ⅲ) Rh(Ⅲ) Pd(Ⅱ) DNA |
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| Abstract: Studies on interactions of Ru(Ⅲ)， Rh(Ⅲ)， Pd(Ⅱ) with DNA have been carried out using berberine(BER)as the probe molecule by fluorescence and UV-Vis absorption spectroscopy. It was found that the fluorescence intensity of BER-DNA system at 526nm was strongly quenched when Ru(Ⅲ) was added to the BER-DNA system; On the con-trary， the fluorescence emission of the system was enhanced when Rh(Ⅲ)， Pd(Ⅱ) were added to it， respectively. Hyperchromicity， spectral red shifts and isosbestic points were observed in absorption spectra of BER-DNA solution in the presence of Ru(Ⅲ)， Rh(Ⅲ)， Pd(Ⅱ) ions. Results show that both Pd(Ⅱ) and Rh(Ⅲ) ions can bind with DNA to form BER-DNA-Metal ion complexes in solution， but Ru(Ⅲ) ion can bind DNA to replace berberine molecules binding to double helix. |
| Keywords: fluorescence probe Ru(Ⅲ) Rh(Ⅲ) Pd(Ⅱ) DNA |
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| 摘要点击次数: 1675 |
| 全文下载次数: 1927 |
| 徐宏娟,于俊生,王树玲,陈洪渊.Ru(Ⅲ)、Rh(Ⅲ)、Pd(Ⅱ)离子与ct-DNA的相互作用研究[J].无机化学学报,2003,19(4):355-360. |
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