| Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ)离子与DNA相互作用的光谱研究 |
| Spectroscopic Studies on the Interactions of Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ) with DNA |
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| 摘要: 本文用中药小檗碱作为探针分子,在0.01mol·L-1醋酸-醋酸钠缓冲体系中,用荧光和紫外-可见吸收光谱研究了贵金属离子Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ)与DNA的相互作用。在三种贵金属离子中,Au(Ⅲ)、Pt(Ⅳ)离子对小檗碱-DNA体系均具有明显的荧光猝灭作用,而Ag(Ⅰ)离子对该体系有强烈的荧光敏化作用。分别求出了三种贵金属离子与DNA的结合常数。三种贵金属离子与DNA结合能力的强弱顺序依次为:Au(Ⅲ) >Ag(Ⅰ) >Pt(Ⅳ)。探讨了三种贵金属离子与DNA的作用机理及导致结合力不同的原因。 |
| 关键词: Ag(Ⅰ) Au(Ⅲ) Pt(Ⅳ) DNA |
| 基金项目: |
| Abstract: The interactions of Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ) ion with DNA were studied by fluorescence spectroscopy and absorption spectroscopy using berberine(BER) as the probe molecule. Obvious spectroscopic changes of the BER-DNA sys-tem in 0.01mol·L-1 HAC-NaAC buffer solution were observed after adding Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ) ion to the binary system, respectively. The apprarent binding constants of these three metal ions with DNA in the BER-DNA binary system were respectively obtained. According to the binding constants obtained, the order for the binding abilities of these three noble metal ions with DNA from the strongest to the weakest was as follows: Au(Ⅲ) >Ag(Ⅰ) >Pt(Ⅳ). Besides, the binding modes of three noble metal ions to DNA as well as the possible causes of different binding abilities of three noble metal ions with DNA were also discussed in this paper. |
| Keywords: Ag(Ⅰ) Au(Ⅲ) Pt(Ⅳ) DNA |
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| 王树玲,于俊生,邹燕辉.Ag(Ⅰ)、Au(Ⅲ)、Pt(Ⅳ)离子与DNA相互作用的光谱研究[J].无机化学学报,2002,18(7):665-670. |
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