| Yb(Ⅲ)、Gd(Ⅲ)与EHPG配合物的光谱研究 |
| Spectral Studies on the Complexes of Yb(Ⅲ) and Gd(Ⅲ) to EHPG |
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| 摘要: 在0.01mol·L-1 N-2-羟乙基哌嗪-N′-2-乙磺酸(Hepes),pH 7.4和室温条件下,应用荧光光谱和紫外差光谱研究了Yb(Ⅲ),Gd(Ⅲ)与N-N′-乙烯-二[2-(2-羟基苯基)甘氨酸](EHPG)的配合反应。结果表明,随着稀土离子Yb(Ⅲ)或Gd(Ⅲ)的不断滴加,EHPG在310nm处的最大荧光峰强度逐渐降低,而其紫外差光谱在238和292nm处的吸收峰逐渐增强,当稀土离子Yb(Ⅲ)或Gd(Ⅲ)达到一定量时,310nm处的荧光强度、238和292nm处的吸收峰强 |
| 关键词: 伴清蛋白 EHPG 镧系离子 紫外差光谱 荧光光谱 |
| 基金项目: |
| Abstract: Under the conditions of 0.01mol·L-1 N-(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid(Hepes)and room temperature and pH 7.4,the reaction process of ethylene-N,N′-bis(o-hydroxyphenylglycine)(EHPG)with Yb(Ⅲ)or Gd(Ⅲ) was monitored by fluorescence spectra and UV difference spectrophotometry. The molar absorptivities for Yb-EHPG and Gd-EHPG complexes were (22.35±0.13)×103cm-1·mol-1·L and (21.64±0.28)×103 cm-1·mol-1·L respectively. When EDTA was added, the UV difference peak at 238nm of Yb-EHPG or Gd-EHPG decreased. Using EDTA as competing agent conditional equilibrium constants of the complexes were lgKYb-EHPG, 15.67±0.13 and lgKGd-EHPG, 13.21±0.23. A linear free energy relationship for the complexes of Yb(Ⅲ) and Gd(Ⅲ) was established by using equilibrium data of 18 complexes. The EHPG binding constants to Yb(Ⅲ) and Gd(Ⅲ) were in accordance with the linear free energy relationship. Under the same conditions, apoovotransferrin (apoOTf) was titrated with Yb(Ⅲ) and Gd(Ⅲ), respectively. Their fluorescent intensities at 338nm was quenched 18%. It means that tyrosine residues in apoovotransferrin coordinate to Yb(Ⅲ) or Gd(Ⅲ) directly. |
| Keywords: ovotransferrin EHPG lanthanide ions fluorescence spectra UV difference spectra |
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| 王金铃,杨斌盛.Yb(Ⅲ)、Gd(Ⅲ)与EHPG配合物的光谱研究[J].无机化学学报,2002,18(6):577-581. |
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