| Raman光谱研究铜锌超氧化物歧化酶及其金属取代衍生物的二级结构 |
| A Study on the Secondary Structures of Copper-Zinc Superokide Dismutase and its Metal-Substituted Derivatives by Raman Spectroscopy |
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| 摘要: 本文测定了铜锌超氧化物歧化酶(Cu2Zn2SOD)及其金属取代衍生物Cu2Ni2SOD的Raman光谱,对图谱进行了归属,并定量测定了两种SOD的二级结构,同时对结构与活性的关系进行了讨论。 |
| 关键词: 超氧化物歧化酶 Raman光谱 二级结构 |
| 基金项目: 国家自然科学基金资助项目(No.29831010) |
| Abstract: The Raman spectra of copper-zinc superoxide dismutase (Cu2Zn2SOD) and its metal - substituted derivative Cu2Ni2SOD were measured and the major bands were assigned.The secondary structure contents of the proteins were obtained quantitatively.The results show that the Cu2Zn2SOD contains 3.0% a-helix, 45.1% β-structure and 5l.9% random coil, which is in agreement with the results obtained by the X-ray crystal analysis and FTIR, and the Cu2Ni2SOD contains 8.2% a-helix, 27.2% β-structure and 64.l% random coil.It can be concluded that the activity of SOD decreases with the decreasing of β-structure and increasing of random coil. |
| Keywords: superoxide dismutae Raman spectroscopy secondary structure |
| 投稿时间：2000-11-08 修订日期：2000-12-25 |
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| 汤银燕,王志林,郁清,耿金龙,罗勤慧.Raman光谱研究铜锌超氧化物歧化酶及其金属取代衍生物的二级结构[J].无机化学学报,2001,17(2):263-268. |
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