| 2,9-二甲基-1,10-菲咯啉的dl-丙氨酸衍生物的合成表征及其镧(Ⅲ)配合物与 DNA作用光谱研究 |
| Synthesis, Characterization of dl-Alanine Derivative of 2, 9-Dimethyl-1, 10-Phenanthroline and DNA-binding Spectral Study of its Lanthanum (Ⅲ) Complex |
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| 摘要: 以2,9-二甲基-1,10-菲咯啉为初始原料,合成了2,9-二甲基-1,10-菲咯啉的dl-丙氨酸衍生物:1,10-菲咯啉-2,9-二亚甲基亚氨基-(2,2'-二甲基)二乙酸(L).该配体经过元素分析。红外光谱、核磁共振氢谱表征。在25±0.1℃、I=0.1mol·dm-3NaNO3的条件下,用pH电位滴定法测定了该配体的质子化常数及其与La(Ⅲ)的配合物的稳定常数。通过电子光谱研究了Lb(Ⅲ)的配合物与小牛胸腺DNA的相互作用。用溴化乙锭作为荧光探针研究了La |
| 关键词: 1,10-菲咯啉 dl-丙氨酸衍生物 La(3Ⅲ)配合物 DNA 光谱 |
| 基金项目: 国家自然科学基金(No．29671020);天津市自然科学基金 |
| Abstract: Starting from 2, 9-dimethyl-1, 10-phenanthroline, its dl-alanine derivative: 1, 10-phenanthroline -2, 9-dimethanoimino- (2, 2'-dimethyl) diacetic acid (L) was synthesized and characterized by elemental analysis, IR, 1HNMR.The protonation constants of the ligand L, the stability constants of La (Ⅲ) -Lbinary complexes have been determined by pHtitration method at 25 ±0.1℃, I= 0.1mol·dm-3 NaNO3. The interaction of the lanthanum (Ⅲ) complex with calf-thymus DNA was studied by electronic spectra.Using ethidium bromide as a fluorescence probe, binding style of the lanthanum (Ⅲ complex with calf-thymus DNA was studied by spectroscopy.For comparison, the interaction of the complexes of lanthanum (Ⅲ) with 1, 10-phenanthroline and dl-alanine with calf-thymus DNA were also studied, respectively.The results indicate that the lanthanum (Ⅲ) complex possibly interacts with calf-thymus DNA by both intercalative and covalent binding. |
| Keywords: 1,10-phenanthroline dl-alanine derivative lanthanum (Ⅲ) complex DNA spectroscopy |
| 投稿时间：1999-12-17 |
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| 汪中明,周志芬,林华宽,朱守荣,刘天府,陈荣悌.2,9-二甲基-1,10-菲咯啉的dl-丙氨酸衍生物的合成表征及其镧(Ⅲ)配合物与 DNA作用光谱研究[J].无机化学学报,2000,16(3):503-509. |
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