| 吡啶、2-甲基吡啶存在下细胞色素c碱式异构化和配体细胞色素c配合物的电化学研究 |
| Electrochemical Study on the Alkaline Isomerization of Cytochrome c and Ligand Binding Cytochrome c in the Presence of Pyridine and 2-methyl- pyridine |
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| 摘要: 用半胱氨酸修饰的金电极研究了吡啶、2-甲基吡啶存在下细胞色素c碱式异构化和配体结合细胞色素c的电化学。在此电极上，细胞色素c可发生准可逆的电极反应而吡啶结合细胞色素c和2-甲基吡啶结合细胞色素c在循环伏安图上只给出还原峰。高浓度（1.27mol·L-1）的吡啶和2-甲基吡啶可诱导碱式细胞色素c在中性条件下生成。进一步的研究表明，这种诱导作用与配体和细胞色素c的键合无关。 |
| 关键词: 循环伏安 细胞色素c 碱式异构化 |
| 基金项目: 国家自然科学基金(No.29731030) |
| Abstract: The alkaline isomerization of horse heart cytochrome c and ligand-binding cyt c in the presence of pyridine and 2-methyl-pyridine has been studied using cyclic voltammetry at a gold disc electrode modified with cysteine over a range of solution pH. At this modified electrode, cyt c shows a quasi-reversible electrode process while the cyclic voltammograms of alkaline cyt c and py· cyt c only give cathodic peaks. At neutral pH, high concentrations (1.27 mol·L-1) of py and 2-mpy caused the formation of alkaline cyt c which appeared only when pH was above 9 in the absence of such ligands. These results confirm the conclusion of previous NMR studies that py and its derivatives induce the formation of the alkaline cyt c at neutral pH. Further investigation indicates that the inducement function is not involved in the ligand's coordination to the heme iron. |
| Keywords: cyclic voltammetry cytchrome c alkaline isomerization |
| 投稿时间：1999-11-25 |
| 摘要点击次数: 1794 |
| 全文下载次数: 1931 |
| 姚勇,鞠先,吴义兵,杨思军,唐雯霞.吡啶、2-甲基吡啶存在下细胞色素c碱式异构化和配体细胞色素c配合物的电化学研究[J].无机化学学报,2000,16(2):346-352. |
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