| 尖吻蝮蛇蛇毒出血毒素Ⅲ的荧光光谱 | |
| FLUORESCENCE SPECTRA OF HEMORRHAGIN Ⅲ FROM THE SNAKE VENOM OF AGKISTRODON ACUTUS | |
| 摘要: 皖南尖吻蝮蛇蛇毒经分离和纯化得到出血毒素Ⅲ(AaH Ⅲ)，质谱测定分子量为23，284.4±0.1。实验表明，Trp残基较大程度位于AaH Ⅲ分子的疏水区。十二烷基磺酸钠(SDS)和盐酸胍的加入使AaH Ⅲ的分子构象发生变化，导致分子内色氨酸(Trp)残基的荧光淬灭。盐酸胍的加入使Trp残基的荧光发射峰位明显红移，表明位于AaH Ⅲ分子较疏水环境的Trp残基相对外露。我们还就酸度、EDTA和金属离子对AaH Ⅲ分子内Trp残基荧光光谱的影响进行了研究和讨论。 | |
| 关键词: 尖吻蝮蛇 出血毒素Ⅲ 荧光光谱 | |
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| Abstract: Hemorrhagin Ⅲ(AaH Ⅲ) was separated and purified from the crude snake venom of Agkistrodon acutus, and its molecule weight was determined accurately to be 23,284.4±0.1 by LDI1700-MALDI-TOF-MS. Emission spectra of AaH Ⅲ showed that Trp residues were located by a great degree in the hydrophobic area. Addition of SDS and guanidine-HCl led to change of the molecular conformation of AaH Ⅲ, and caused the fluorescence quenching of Trp residues. The red-shifted emission band of AaH Ⅲ after adding guanidine-HCl showed that Trp residues exposed in polar solvents. The effects of pH, EDTA and metal ions on the fluorescence spectra of AaH Ⅲ were also investigated. | |
| Keywords: Agkistrodon acutus hemorrhagin Ⅲ fluorescence spectra | |
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| 陈真龙,赵大庆,刘清亮,丁兰,张祖德,倪嘉缵.尖吻蝮蛇蛇毒出血毒素Ⅲ的荧光光谱[J].无机化学学报,1999,15(2):185-190. | |
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