| [Cu(dien)Cl]+与核苷酸作用的1H、31P NMR研究 |
| 1H and 31P NMR STUDIES OF THE INTERACTION OF [Cu(dien)Cl]+ WITH NUCLEOTIDES |
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| 摘要: 本文用1H-NMR和31P-NMR谱研究了[Cu(dien)Cl]+与5'-AMP、5'-GMP和5'-CMP在pD=6.00条件下的共价键合作用.氢核磁共振谱研究表明[Cu(dien)]2+与5'-AMP可以在N-7和N-1位上键合,但N-7是最有利的键合位置.配离子与5'-GMP、5'-CMP、分别键合在N-7和N-3位置上.31P核磁共振谱研究结果则表明[Cu(dien)]2+还可以与单核苷酸上的磷酸酯根键合.并根据显著加宽质子峰及31P峰的最低[Cu(dien)Cl]+浓度,比较了[Cu(dien)]2+对三种核苷酸的碱基与磷酸酯根的亲力. |
| 关键词: 铜(Ⅱ) 核苷酸 1H核磁共振 31P核磁共振 |
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| Abstract: The interaction of [Cu(dien)Cl]+ with 5'-AMP, 5'-GMP, and 5'-CMP at pD = 6.0 have been studied by 1H and 31P-NMR spectroscopy. The broadening effect of [Cu(dien)Cl]+ on the 1H peaks of nucleotides reveal [Cu(dien)]2+ binding to N-7 of 5'-GMP and N-3 of 5'-CMP respectively, and binding 5-AMP at N-7 and N-l simultaneously, but N-7 is the mere preferential position. The 31P-NMR spectra indicate that [Cu(dien)]2+ can also bind with phosphate of mentioned nucleotides. Comparing the lowest concentration of [Cu(dien)Cl]+, at which the apparent broadening of proton and 31P peaks occur, the affinity of [Cu(dien)]2+ to the Natoms on bases and the phosphate was discussed. |
| Keywords: copper(Ⅱ) nucleotide 1H-NMR 31P-NMR |
| 投稿时间：1991-03-07 |
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| 赵刚,吴仲岿,管荫桐,唐雯霞.[Cu(dien)Cl]+与核苷酸作用的1H、31P NMR研究[J].无机化学学报,1993,9(1):7-11. |
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